Antifungal Activity of Plant-Derived Essential Oils on Pathogens of Pulse Crops.

Abstract

Pulse crops such as chickpeas, lentils, and dry peas are grown widely for human and animal consumption. Major yield- and quality-limiting constraints include diseases caused by fungi and oomycetes. The environmental and health concerns of synthetic fungicides used for disease management, emergence of fungicide-resistant pathogens, and demand for organic pulse crop products necessitate the search for effective alternatives. Safe and environmentally friendly plant-derived essential oils (EOs) have been reported effective against some pathogenic fungi. Growth on EO-amended growth medium and an inverted Petri plate assay were used to determine the effects of 38 oils and their volatiles on mycelial growth and spore germination of important pathogenic fungi and oomycetes: Aphanomyces euteiches, Botrytis cinerea, Colletotrichum lentis, Didymella pisi, D. rabiei, D. lentis, Fusarium avenaceum, Stemphylium beticola, Sclerotinia sclerotiorum, and Pythium sylvaticum. Palmarosa, oregano, clove, cinnamon, lemongrass, citronella, and thyme oils incorporated in media inhibited mycelial growth of all the pathogens by 100% at 1:1,000 to 1:4,000 dilution. In addition, thyme oil (1:500 dilution) showed complete inhibition of conidial germination (0% germination) of F. avenaceum and D. pisi. All seven EO volatiles inhibited mycelial growth of all pathogens by 50 to 100% except for B. cinerea and S. sclerotiorum. EO effects on mycelial growth were fungistatic, fungicidal, or both and varied by EO. EOs show potential for management of major crop diseases in organic and conventional production systems.