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https://doi.org/10.3389/conf.fmars.2014.02.00048
Copy DOIJournal: Frontiers in Marine Science | Publication Date: Jan 1, 2014 |
Citations: 1 | License type: cc-by |
Event Abstract Back to Event Antifungal activity of low molecular weight chitosan produced from non-traditional marine resources Francisco P. Avelelas1*, Luís F. Pinto2, Cátia Velez3, Sarah Azinheiro1, Américo Rodrigues1 and Sérgio M. Leandro1 1 Escola Superior de Turismo e Tecnologia do Mar, GIRM - Marine Resources Research Group, Portugal 2 Universidade Nova de Lisboa, CENIMAT/I3N, Departamento de Ciências dos Materiais, Portugal 3 Universidade de Aveiro, Departamento de Biologia & CESAM, Portugal The four plants pathogens, Botrytis cinerea, Phytophthora cinnamomi, Cryphonectria parasitica and Heterobasidion annosum are responsible for several diseases affecting different plant species in Portugal, such as pines (H. annosum), chestnuts (P. cinnamomi and C. parasitica) and eucalyptus (B. cinerea). These pathogens incurs in large economic losses, and ultimately causes the death of these plants. The use of biopolymers as antimicrobial agents, such as chitosan (derived from chitin), is increasing, in order to reduce the negative impact of conventional chemical treatments on the environment, avoiding health risks. Therefore, eco-friendly polymers were produced through (1) N-acetylation with addition of acetic anhydride and (2) hydrogen peroxide of chitosan samples, obtained from two different sources: shrimp (commercial chitosan) and swimming crab bycatch specie Polybius henslowii. The chemical structure and molecular weight of the prepared chitosan derivatives, water soluble chitosan (WSC) and chitooligosaccharides (COS), was confirmed by Fourier Transform Infrared (FT-IR) and Gel Permeation Chromatography (GPC) and their antifungal activity evaluated against Botrytis cinerea, Phytophthora cinnamomi, Cryphonectria parasitica and Heterobasidion annosum. The concentration range varied from 0.0125 to 0.1 mg/mL and inhibition percentages were determined by differences in radial growth on the agar plates for all species. Although not all species tested exhibited equal vulnerability towards the concentrations range, antifungal activity of chitosan samples proved to be dependent, increasing the inhibitory capacity with lower concentrations. The results obtained support the use of chitosan fromPolybius henslowii when compared with commercial chitosan with shrimp towards antifungal approaches, suggesting that chitin producers can rely on this crab waste as a raw material for chitin extraction, adding value to this bycatch specie. Financial support was obtained from European Fisheries Fund – Axis 4 managed by Fishery Local Action Group Oeste-Portugal. Keywords: Chitosan, Antifungal activity, Chitooligosaccharide, marine resources, Polybius henslowii Conference: IMMR | International Meeting on Marine Research 2014, Peniche, Portugal, 10 Jul - 11 Jul, 2014. Presentation Type: Poster Presentation Topic: BLUE BIOTECH Citation: Avelelas FP, Pinto LF, Velez C, Azinheiro S, Rodrigues A and Leandro SM (2014). Antifungal activity of low molecular weight chitosan produced from non-traditional marine resources. Front. Mar. Sci. Conference Abstract: IMMR | International Meeting on Marine Research 2014. doi: 10.3389/conf.fmars.2014.02.00048 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 08 May 2014; Published Online: 18 Jul 2014. * Correspondence: Ms. Francisco P Avelelas, Escola Superior de Turismo e Tecnologia do Mar, GIRM - Marine Resources Research Group, Peniche, 250-641, Portugal, franciscoavelelas@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Francisco P Avelelas Luís F Pinto Cátia Velez Sarah Azinheiro Américo Rodrigues Sérgio M Leandro Google Francisco P Avelelas Luís F Pinto Cátia Velez Sarah Azinheiro Américo Rodrigues Sérgio M Leandro Google Scholar Francisco P Avelelas Luís F Pinto Cátia Velez Sarah Azinheiro Américo Rodrigues Sérgio M Leandro PubMed Francisco P Avelelas Luís F Pinto Cátia Velez Sarah Azinheiro Américo Rodrigues Sérgio M Leandro Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.
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