Antifungal activity of leaf extract of Mansoa alliacea against Colletotrichum acutatum the cause of anthracnose disease on chili pepper

Abstract

Antifungal activity of the leaf extract of Mansoa alliacea against Colletotrichum acutatum the cause of anthracnose disease on chili pepper was be done. The antifungal activity was determined based on the effects of plant extract to the fungal growth, spore’s formation, spore’s germination and total biomass. Results of this study showed that the crude extract of Mansoa alliacea obviously inhibited the growth of Colletotrichum acutatum on PDA with MIC by 0.7% (w/v). Treatment with leaf extract of Mansoa alliacea significantly (P<0.05) inhibited the fungal growth, spore’s formation, spore’s germination and fungal biomass of Colletotrichum acutatum. The inhibitory activity for the colony radial growth, spore’s formation, spore’s germination, and fungal biomass were respectively ranged from 29.72 to 81.11%, 63.18 to 100%, 61.68 to 100% and 39.53 to 91.86%. This result suggested that the leaf extract of Mansoa alliacea contains the antifungal susbtances that responsable for the antifungal activity such as phenol and alkaloid, so this extract can be considered as one of alternative measures to control the anthracnose disease on chili pepper.