Antifungal Activity of Culture Filtrate from Endophytic Fungus Nectria balsamea E282 and Its Fractions against Dryadomyces quercus-mongolicae

Abstract

A key role that fungal endophytes play in interacting with their host plant can be defined by the fact that they promote the growth of plants and enhance the tolerance of the host against plant pathogens using bioactive compounds that they produce. Several studies utilizing endophytic fungi as a source of biological control against plant pathogens were conducted, and a representative example includes Aureobasidium protae from common wheat (Triticum aestivum), which inhibited the mycelial growth of Fusarium graminearum and Fusarium culmorum, causal agents of Fusarium head blight disease. Our previous study indicated that three endophytic fungal isolates, E089 (Daldinia childiae), E282 (Nectria balsamea), and E409 (Colletotrichum acutatum), showed antifungal activities against D. quercus-mongolicae, an ascomycetous fungus that is reported to be associated with oak mortality in South Korea. The objectives of this study were to optimize and evaluate antifungal efficiency for these endophytic fungi against D. quercus-mongolicae, and this was achieved using culture filtrate retrieved from the three above-mentioned endophytes and fractions isolated from the culture filtrate. Of those, the culture filtrate from E282 showed higher mycelial growth and sporulation inhibitions on PDA medium where D. quercus-mongolicae was grown. In addition, three fractions, including hexane, CHCl3, Et2O, and H2O, were tested for antifungal activities against D. quercus-mongolicae. The results revealed that the Et2O fraction showed higher mycelial growth and sporulation inhibition rates. Taking these results together, the endophytic fungus, N. balsamea, which exhibited high antifungal efficiency, can be effectively used as a biocontrol agent for the management of oak wilt disease in the country.