Antifungal activity of cow urine distillates of local botanicals against major pathogens of bell pepper

Abstract

Cow urine distillates of botanicals viz, Ranunculus muricatus, Melia azedarach, Vitex negundo, Eupatorium sp. and Murraya koenigii and cow urine alone were evaluated at 0.5, 2.0, 4.0, 6.0, 8.0 and 10.0% concentrations under in vitro conditions against bell pepper pathogens namely Rhizoctonia solani, Sclerotinia sclerotiorum, Sclerotium rolfsii, Phytophthora nicotianae, Fusarium oxysporum f.sp. capsici, Fusarium solani and Colletotrichum capsici. M. koenigii and V. negundo provided the complete inhibition in mycelial growth of C. capsici, Rhizoctonia solani, Phytophthora nicotianae, Sclerotinia sclerotiorum, Fusarium solani and S. rolfsii at 10% concentration. R. muricatus and Murraya azedarach caused 100% inhibition of R. solani, S. sclerotiorum and S. rolfsii whereas more than 98% inhibition in mycelial growth of F. solani, P. nicotianae, R. solani and C. capsici was observed at 10% concentration. Eupatorium sp. showed 63.05 to 99.69% inhibition against all the test pathogens. Inhibition in rest of the concentrations ranged between 85.06 to 98.97%. Cow urine distillate exhibited more than 98% inhibition in mycelial growth of P. nicotianae and S. sclerotiorum and 93% inhibition of C. capsici, R. solani, F. solani, F. oxysporum f.sp. capsici and S. rolfsii at 10% concentration and inhibition in rest of concentrations was observed to the tune of 59.70 to 96.77%. Cow urine distillates of botanicals were found more inhibitory to pathogens than cow urine distillate alone. Key words: Antifungal activity, cow urine distillate, Ranunculus muricatus, Melia azedarach, Vitex negundo, Eupatorium sp., Murraya koenigii.