Abstract
Biological control with bioagents is a cost effective alternate method for the management of crop diseases. The antagonistic bacterial strains were explored for the management of leaf blight of marigold which is caused by Alternaria alternata . The present study clearly proved that the mycelial growth of A. alternata was inhibited up to 83% by Bacillus subtilis subsp. spizizenii (MM19) in vitro . GC/MS analysis of partially purified extracts of B. subtilis subsp. spizizenii (MM19) revealed the presence of antifungal Phthalic acid esters which might be responsible for the inhibition of the pathogen. Foliar application of B. subtilis subsp. spizizenii (MM19) under field conditions suppressed leaf blight by 77%. This study highlighted the potential of B. subtilis subsp. spizizenii (MM19) for the management of Alternaria leaf blight.
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