Abstract
We investigated the effect of a recombinant cystatin (proteinaceous cysteine proteinase inhibitor) cloned from carnation and expressed in Escherichia coli (rDC-CPIn) on the growth of phytopathogenic fungi. rDC-CPIn inhibited the growth of Phytophthora nicotianae, Botrytis cinerea, and Sclerotiana sclerotiorum. These results suggest that the cDNA for carnation cystatin could be useful for the generation of transgenic plants with increased defenses against fungal plant pathogens.
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