Abstract
An antifungal compound was isolated from Aspidistra elatior Blume. The methanol extract showed antifungal activity against Saccharomyces cerevisiae, Hansenula anomala, Mucor mucedo, and Candida albicans. The powdered methanol extract was dissolved in water and partitioned with hexane, ethyl acetate, n-butanol, and then water. Only the n-butanol fraction exhibited antifungal activity. The antifungal compound in the n-butanol fraction was finally isolated by silica gel chromatography and HPLC. Analysis by fast-atom bombardment mass spectroscopy and Fourier-transform nuclear magnetic resonance spectroscopy revealed that the structure of the antifungal compound is aspidistrin (diosgenin 3-O-β-lycotetraoside). The minimum inhibitory concentration of aspidistrin was 2.5 μg/mL against S. cerevisiae, 10 μg/mL against H. anomala, 10 μg/mL against M. mucedo, and 50 μg/mL against C. albicans. This is the first report of antifungal activity against food-borne fungi of aspidistrin. Keywords: Aspidistra elatior Blume; aspidistrin; antifungal activity
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
