Antifungal Activities Of Cinnamon Leaf Extracts Against Sigatoka Fungus (Pseudocercospora Fijiensis)

Abstract

Efforts to reduce the negative impacts caused by the use of synthetic fungicides need to be done in implementing environmentally sustainable agriculture. The use of biofungicides should be done. The acetone extract of cinnamon leaves has the potential as a biofungicide. Based on this, a series of experiments were carried out: crude cinnamon extract test against the test fungus, i.e. the fungus causing Sigatoka disease in banana plants, MIC (Minimum Inhibiting Concntration) extract, effectiveness test of extract on colony, biomass, spore formation, and identification of cinnamon leaf extract content based on partition, column chromatography and GS-MS analysis. The rough extract of cinnamon leaves inhibited the growth of the Sigatoka fungi (P. fijiensis) in-vitro on PDA media with a diameter of 30 mm which means that this extract has a very strong inhibitory activity, with a MIC value of 0.5% (w/v). Treatment of extracts with concentrations between 0.1 and 0.5% significantly (P <0.05) inhibited the growth of fungal colonies, fungal biomass and fungal spore formation. There were 16 compounds found in the active fraction of cinnamon leaf extracts and 3 dominant compounds that are anti-fungal compounds, namely 1.2-Benzenedicarboxylic acid, mono 2-ethyl (29%), 2H-I-Benzopyran-2- one (CAS) Coumarin (11.9%) and 2,6-Dimethyl-6-nitro-2-hepten-4-one (11.5%).