Abstract
Serratia plymuthica strain UBCF_13 is a potential biocontrol agent to inhibit pathogen causing anthracnose disease in plants, Colletotrichum gloesporioedes. The production of bacterial antifungal compounds is affected by nutrition and the growth phase of bacteria. This study is aimed to determine the optimal medium and duration of culture for the production of antifungal compounds. This study used three types of liquid media culture for UBCF_13 [King’s B, Potato Dextrose, and tryptic Soy] and six variations in duration culture [8, 16, 24, 32, 40, and 48 hours]. Extracellular that has been produced was used for antifungal activity assay against C. gloeosporioides. The results showed that the extracellular produced in the PDB with 48 hours culture duration could inhibit 18%. C. gloeosporioides growth. Different nutritional compositions for UBCF_13 cultures show affect the metabolism and compound produced as well. This was indicated by differences in protein profiles of the intracellular and extracellular. Further studies are necessary to be carried out, particularly to see the mechanism of the nutrient medium that could affect the metabolism of UBCF_13 in the synthesis of antifungal compounds.
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