Antifouling activity of aquatic macrophyte extracts on estuarine bacterial biofilms

Abstract

ABSTRACT Ecologically safe antifouling solutions have received growing attention since the acknowledgment of the environmental risks of traditional biocides used currently. Given that bacteria constitute the initial stage of the biofouling process, this work aims to evaluate the antifouling potential of aquatic macrophyte extracts in inhibiting biofilm-forming bacteria and to evaluate their ecotoxicological effects on target and nontarget organisms (Thalassiosira pseudonana and Nitokra sp.). For this, the ability of 25 macrophyte extracts to inhibit and eradicate single and multispecies estuarine biofilms was evaluated. The extracts and respective serial dilutions that showed a biofilm inhibitory effect ≥ 60% were evaluated in ecotoxicological assays. Of the 25 extracts, only 6 extracts inhibited the biofilm ≥ 60%. The extracts of Cabomba caroliniana and Schoenoplectus californicus stood out as having a biofilm inhibitory effect ≥ 80% (p < 0.05) for bacterial isolates and ≥ 70% (p < 0.05) for multispecies biofilms. Furthermore, these extracts showed no toxic effects on nontarget organisms. These results demonstrate the biotechnological potential of macrophyte compounds.