Abstract
In order to promote the development and application of small molecule oligopeptides from tilapia (SMOT), its anti-fatigue effect was evaluated by using exhaustive swimming in mice. The results showed that compared with the negative control group, SMOT could significantly prolong the exhausted swimming time of mice, reduce blood lactic acid, blood urea nitrogen and malondialdehyde content, and could significantly improve liver glycogen storage status. Meanwhile, the activities of superoxide dismutase and glutathione peroxidase of the mice gavaged with SMOT were significantly higher than those in the negative control group. In addition, SMOT could increase the activity of immune factor IL-2, stimulate the secretion of sIgA and regulate the level of inflammatory factors. So SMOT had significant anti-fatigue effect. Its anti-fatigue effect was exerted by increasing energy reserves, eliminating the accumulation of metabolites, reducing oxidative stress, and improving the body’s immune function.
Highlights
Fatigue is defined as a feeling of discomfort, and it will lead to inability to complete original normal activities or work under the same conditions
Papain (2 × 105 U/g) was purchased from Pangbo Bioengineering Co., Ltd.(Nanning, China); Acetonitrile (HPLC grade) was produced from Sigma, USA; Glutathione was bought from Solarbio Technology Co., Ltd.( Beijing, China); Interleukin-2 (IL-2), interleukin-10(IL-10), interleukin-1β (IL-1β), interferon-γ (IFN-γ), tumor necrosis factor-a (TNF-a), secretory immunoglobulin A and Caspase-3(Casp-3) ELISA kit were all obtained from Jiangsu Enzyme Biotechnology Co., Ltd.(Jiangsu, China); Kits for analysis of blood lactic acid (BLD), blood urea nitrogen (BUN), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), malondialdehyde ( MDA), liver glycogen (LG) were all got from Nanjing Jiancheng Biotechnology Institute (Nanjing, China); All the other chemical reagents were of analytical grade
The small molecule peptide in the range of 180-1000 Da accounted for about 96% of small molecule oligopeptides from tilapia (SMOT) (Data was not shown in this article)
Summary
Fatigue is defined as a feeling of discomfort, and it will lead to inability to complete original normal activities or work under the same conditions. Chemical drugs have been a main treatment to relieve fatigue These drugs have adverse effects on the nervous system and normal physiological activities (Itzhak & Ali, 2006). In this study, taking mouse swimming as an animal model, the anti-fatigue effect of small molecule oligopeptides from tilapia (SMOT) was discussed by recording the exhausting swimming time of mice, measuring the fatigue related biochemical parameters and immune related parameters, so as to provide reference for the development of high efficacy, low cost, safe and healthy anti-exercise fatigue food
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