Abstract

Purpose: To study the anti-endometriotic effect of Angelica sinensis (Oliv.) Diels extract (ASDE) in human endometriotic cells and rats.Method: Forty female rats were randomly divided into four groups (10 rats/group): control, endometriosis+danazol, endometriosis+high dose of ASDE and low dose of ASDE. The rats were orally administered either vehicle (200 μL of PBS) alone or ASDE (140, 280 and 560 mg/kg/day) for 5 weeks. Danazol was used as the control drug. After induction of endometriosis for 4 weeks, the rats were sacrificed by cervical dislocation and the peritoneum and visceral organs examined visually to measure the number of endometriotic lesions. Serum levels of cancer antigen 125 (CA-125) and interleukin 13 (IL-13), interleukin 18 (IL-18) and tumor necrosis factor-alpha (TNF-α) of peritoneal fluids of rats were measured using ELISA kits. Western blot assay was performed to measure the levels of matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) expressions after 24 h of treatment with ASDE (30, 60, and 120 μg/mL).Results: ASDE-treated rats displayed reduced numbers of total endometriotic lesions when compared with vehicle-treated controls (p < 0.01). When the rats were treated with high dose of ASDE, serum CA-125 level, as well as IL-18 and TNF-α levels in peritoneal fluids were significantly lower than that of the control group (p < 0.01); however, IL-13 level in peritoneal fluids was significantly higher than that of the control group (p < 0.01). ASDE treatment significantly suppressed the levels of MMP-2 and MMP-9 protein in 11Z cell (p < 0.01).Conclusion: The results reveal that ASDE exhibits significant anti-endometriotic effect by inhibiting inflammatory factors in rats. Thus, the plant extract can potentially be developed for the clinical management of endometriosis.
 Keywords: Angelica sinensis, Endometriosis, Cancer antigen, Endometriotic lesions, Matrix metalloproteinase

Highlights

  • Endometriosis is a common gynaecological disease that affects approximately 1 in 7 women and 30 - 50 % of infertile women [1]

  • Increased peritoneal fluid concentrations of cytokines that lead to the migration, proliferation, and activation of macrophages have been reported in patients with endometriosis

  • Angelica sinensis (Oliv.) Diels extract (ASDE) inhibited the formation of endometriosis-like lesions in rats

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Summary

INTRODUCTION

Endometriosis-associated inflammatory response, tissue repair and neo-vascularization are dependent on the peritoneal fluids macrophages and their secretary products/cytokines [4]. These cytokines may play major roles in regulating cell proliferation, activation, motility, adhesion, chemotaxis and morphogenesis in the pathogenesis of endometriosis [5]. Increased peritoneal fluid concentrations of cytokines that lead to the migration, proliferation, and activation of macrophages have been reported in patients with endometriosis. The anti-endometriotic effect of ASDE was investigated in human endometriotic cells and rats. The medicinal herbs of Angelica sinensis (Oliv.) Diels weighing 5 kilogram were collected from Guilin City, Guangxi Province in China in October 2018. For comparing more than two groups, one-way analysis of variance (ANOVA) was used followed by Tukey test with statistical significance set at p < 0.05

RESULTS
DISCUSSION
Conflict of interest

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