Abstract
To examine the acanthamoebicidal effects of ethyl acetate, aqueous and butanol fractions of dried flower buds of Lonicera japonica (L. japonica) Thunb. (Flos Lonicerae) invitro. Acanthamoeba triangularis isolates were obtained from environmental water samples and identified by PCR. They were exposed to ethyl acetate, water and butanol fractions of L.japonica Thunb. at concentrations ranging from 0.5mg/mL to 1.5mg/mL. The extracts were evaluated for growth inhibition at 24, 48 and 72h, respectively. Chlorogenic acid at a concentration of 1mg/mL was examined for inhibition of encystment. Ethyl acetate fraction at a concentration of 1.5mg/mL evoked a significant reduction of trophozoite viability by 48.9% after 24h, 49.2% after 48h and 33.7% after 72h chlorogenic acid, the major active constituent of L.japonica Thunb. at the concentration of 1mg/mL reduced the cysts/trophozoite ratio by 100% after 24h, 84.0% after 48h and 72.3% after 72h. This phenolic compound at concentration of 1mg/mL concurrent with 0.6% hydrogen peroxide inhibited hydrogen peroxide-induced encystment by 92.8% at 72h. Results obtained from this study show that ethyl acetate fraction at 1.5mg/mL is the most potent fraction of L.japonica Thunb. and its major constituent chlorogenic acid showed the remarkable inhibition of encystment at a concentration of 1mg/mL.
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