Abstract
This study was carried out to explore the mechanism underlying antidiarrheal and antispasmodic activities of Vincetoxicum stocksii. The crude extract of V. stocksii provided 12-24% protection from castor oil-induced diarrhea at the dose of 300-1,000 mg/kg, similar to loperamide. In isolated rabbit jejunum preparations, V. stocksii caused inhibition of the spontaneous and high K+ (80 mM)-induced contractions, with respective EC50 values of 2.53 (1.65-3.90) and 0.95 mg/mL (0.63-1.42), similar to that caused by verapamil, suggesting the calcium channel blocking effect. Loperamide caused inhibition of spontaneous and high K+-induced contraction, with respective EC50 values of 8.59 (6.33-10.11) and 9.12 µM (7.33-12.81). The calcium channel blocking activity was further confirmed when pretreatment of the tissues with V. stocksii (1-3 mg/mL) caused a rightward displacement of the Ca++ concentrations response curves, similar to that produced by verapamil; constructed in Ca++ free medium. These data indicate that the crude extract of V. stocksii contains calcium channel blocking constituents that may possibly explain its medicinal use in hyperactive states of gut, such as, diarrhea and spasms.
Highlights
Vincetoxicum stcoksii Ali and Khatoon belongs to the Asclepiadaceae family and is a group of perennial climbing leafy vines (Zaidi and Crow, 2005)
The crude extract of V. stocksii was tested for its antidiarrheal activity
In the castor oil-induced diarrhea in mice, the crude extract of V. stocksii, like loperamide, a standard antidiarrheal agent (Reynolds et al, 1984), inhibited significantly (p
Summary
Vincetoxicum stcoksii Ali and Khatoon belongs to the Asclepiadaceae (milkweed) family and is a group of perennial climbing leafy vines (Zaidi and Crow, 2005). When the control concentration-response curves of Ca++ were found super-imposable (usually after two cycles), the tissue was pretreated with the plant extract for 60 min to test the possible calcium channel blocking effect.
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
