Abstract
Most of every part of bamboo plants had economical value. The wood is used as building and furniture, the shoot is processed as health foods and medicines, t he root and culms are used as traditional medicine. The leaf has been believed that it could heal diarrhea in pi glets, rabbit, poultry and calves. This research wa s designed to investigate the inhibition activity of apus bamboo leaf extracts against four strains pathogen ic Escherichia coli . The leaf of apus bamboo ( G. apus ) was extracted in methanol, ethanol and methanol-e thanol (1:1), subsequently dried and assayed for their antibacter ial activity using diffusion and dilution. Among th ree solvents used in this study, ethanol was the best w ith a yield of 18.74% and its effectivity was about 0.44% compared to tetracycline. The bioactive compounds in the extract were fatty acids, esters and alcohols .
Highlights
Plants have been used as the source of functional foods and drugs (Ciocan and Bara, 2007)
Four strains of E. coli used as tested bacteria were two strains isolated from diarrheal piglet (Veterinary Research Institute, Bogor) and poultry (Gadjah Mada University, Yogyakarta) and E. coli O157:H7 isolated from well water and river water, obtained from Culture Collection, Microbiology and Fermentation Laboratory, Faculty of Biotechnology Atma Jaya Catholic University (Jakarta)
Antibacterial activity assay using well and disc diffusion methods could not result in definite clear zone
Summary
Plants have been used as the source of functional foods and drugs (Ciocan and Bara, 2007). Many reports have been published about natural antimicrobial agents from plants and microbiota origin. The aqueous extract of edible mushroom (Dictyophora indusiata) had antimicrobial activities against several pathogenic or putrefactive bacteria and fungi, such as Escherichia coli, Alcaligenes faecalis, Salmonella typhimurium, Shigella dysenteriae, Pseudomonas aeruginosa, Bacillus subtilis, Staphylococcus aureus, B. cereus, Aspergillus flavus, Candida albicans and Cryptococcus neoformans and among all tested microbiota, only A. niger that was resistant (Oyetayo et al, 2009). The fruits of Phyllanthus acidus, Punica granatum, Citrus aurantifolia and C. aurantium had inhibitory effect against E. coli. The leaf of Tamarindus indica and Bamboosa arundinaceae had inhibitory effect against E. coli (Melendez and Capriles, 2006; Singh et al, 2010)
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