Abstract
Nagasari (Mesua ferrea L.) is empirically used for curing diarrhea in Nusa Tenggara Barat Province. However, the safety and efficacy of nagasari have not been scientifically validated in animal model. This study evaluates the activity of nagasari flowers aqueous extract in BALB/c mice induced by Escherichia coli to determine the most effective dosage for anti-diarrheal effect. The aqueous extract of nagasari flowers ((0.4% (w/v), 0.8% (w/v), and 1.6% (w/v)) was given to 3 groups of mice whereas negative and positive control groups received aquadestilate and ciprofloxacin 130 mg/kgBW respectively orally once per day for 3 days. The frequency and consistency of feces were observed then the total colony of E. coli in feces was calculated using colony counter. Aqueous extract of nagasari flowers at concentration of 1.6% showed significant inhibitory activity against Escherichia coli compared to positive controls (diarrhea index of 0.425; p>0.05 and total colony of E. coli in feces of 100.2; p>0.05). This study provides scientific support for the traditional use of aqueous extract of nagasari flowers for the treatment of diarrheal diseases.
Highlights
Diarrhea is an abnormal event that characterized by an increased in the frequency of defecate more than 3 times in 24 hours accompanied by the consistency of liquid stool (Kotloff et al, 2013).Based on the Health Profile in Nusa Tenggara Barat (NTB) Province, the number of diarrhea patients had not experienced a significant decrease that is 158.993 and 175.361 in the year of 2015 and 2017 (Dinkes RI, 2015; 2018)
Diarrhea can be caused by infectious agents such as bacteria, viruses and parasites, including Escherichia coli which includes Gram-negative bacteria
The AEONF (Table I and Figure 2) at concentration 1,6% showed the highest decreasing in the total colony of Escherichia coli compared to other concentration and the antibacterial effect compared to positive controls (p>0,05)
Summary
Diarrhea is an abnormal event that characterized by an increased in the frequency of defecate more than 3 times in 24 hours accompanied by the consistency of liquid stool (Kotloff et al, 2013).Based on the Health Profile in Nusa Tenggara Barat (NTB) Province, the number of diarrhea patients had not experienced a significant decrease that is 158.993 and 175.361 in the year of 2015 and 2017 (Dinkes RI, 2015; 2018). 1. In vitro, the methanol extract of all parts of the nagasari flowers at concentration of 10 μg/mL, 50 μg/mL and >200 μg/mL showed the minimum inhibitory concentration values of E. coli of 2, 6 and 14 strains. In vivo scientific studies of nagasari flower extract as anti-diarrhea are still limited.
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