Antidiabetic effects of marine brown algae Spatoglossum asperum J. Ag. ethanolic extract on 3T3-L1 Pre-Adipocytes activity an in vitro approach

Abstract

Marine algae have emerged as good alternative source of medical, pharmaceutical, and food industries, it’s used for anticancer, anti-inflammatory, antioxidant, antimicrobial, etc., In this study, anti-diabetic effect of brown seaweed Spatoglossum asperum was focused, We aimed systematically to examine the ethanolic extract effects of S. asperum (EESA) as an anti-diabetic agent using 3T3-L1 in vitro assay, focused through molecular works; protein extraction, DNA isolation, gene analysis, western blot, ROS measurement, senescence associated β-galactosidase and deoxy glucose uptakes. In results for p21/β-actin H2O2+EESA treated cells showed very good potential results showed (0.44), same combination reduced the toxic level (0.60) in p53/β-actin, awfully superior results showed (0.39) in p16/β-actin. In adiponectin (1.16), tnf-α (0.68), il6 (0.42), RFU (211.5), same combination in COMET assay observed that DNA damage (4.95), glucose uptake noticed (68.45%), mRNA expression in p53 showed (0.07), p21 (1.8), adiponectin (3.25), tnf-α obtained (1.95), il6 expression obtained (2), p16 expression (2). From the results concluded that the capability to supplement glucose absorption in 3T3-L1 pre-adipocytes and augment glucose consumption activity through the appearance of glucose transporter type 4 (GLUT 4).