Abstract
The objective of the present study was to evaluate α-glucosidase inhibitory and antidiabetic effects of Nopal water extract (NPWE) and Nopal dry power (NADP) in low-dose streptozotocin- (STZ-) induced diabetic rats fed a high-fat diet (HFD). The type 2 diabetic rat model was induced by HFD and low-dose STZ. The rats were divided into four groups as follows: (1) nondiabetic rats fed a regular diet (RD-Control); (2) low-dose STZ-induced diabetic rats fed HFD (HF-STZ-Control); (3) low-dose STZ-induced diabetic rats fed HFD and supplemented with NPWE (100 mg/kg body weight, HF-STZ-NPWE); and (4) low-dose STZ-induced diabetic rats fed HFD and supplemented with comparison medication (rosiglitazone, 10 mg/kg, body weight, HF-STZ-Rosiglitazone). In results, NPWE and NADP had IC50 values of 67.33 and 86.68 μg/mL, both of which exhibit inhibitory activities but lower than that of acarbose (38.05 μg/mL) while NPWE group significantly decreased blood glucose levels compared to control and NPDP group on glucose tolerance in the high-fat diet fed rats model (P < 0.05). Also, the blood glucose levels of HR-STZ-NPWE group were significantly lower (P < 0.05) than HR-STZ-Control group on low-dose STZ-induced diabetic rats fed HFD. Based on these findings, we suggested that NPWE could be considered for the prevention and/or treatment of blood glucose and a potential use as a dietary supplement.
Highlights
Diabetes is a disease that seriously threatens human health, with more than 200 million people suffering from the disease worldwide
The results of this study suggested that cactus pads reduce absorption of water-soluble dietary fiber content by interrupting absorption of glucose in the intestine and showed reduced blood glucose levels after ingestion of Opuntia ficus-indica, with this reduction reaching a statistically significant level after 120–180 min [7]
Collected dried powder (NPDP) was ground into ultrafine particles, and stems and distilled water were put into an ultrahigh-speed low-temperature vacuum extractor, which was maintained at 80–90∘C for 3 h to produce water extract powder of fresh NP stem (NPWE)
Summary
Diabetes is a disease that seriously threatens human health, with more than 200 million people suffering from the disease worldwide. Trejo-Gonzalez et al assessed the blood glucose-reducing activity of purified extract from prickly pear cactus (Opuntia sp.) in streptozotocin- (STZ-) induced diabetic mice [8]. They reported that the mechanism of action is not known, the major substance that reduces blood glucose is presumed to be the dietary fiber in Opuntia extract (1 mg/kg body weight/d). The purpose in our study was to investigate the evaluation for the prevention and treatment of antidiabetic effect from water extracts of fresh NP stem on the blood glucose control effect by conducting glucose tolerance experiments in the STZinduced diabetic animal model fed a high-fat diet, as well as the α-glucosidase activity in vitro and dietary fiber contents
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
