Abstract

In order to evaluate the antioxidant activity of evaporated ethanolic extract (EESB) and lyophilized water extract (WESB) of Shaggy bindweed (Convulvulus betonicifolia Mill. Subs), some putative antioxidant methods such as DPPH· scavenging activity, ABTS•+ scavenging effect, ferric ions (Fe3+) reduction method, cupric ions (Cu2+) reducing capacity, and ferrous ions (Fe2+) binding activities were separately performed. Also, ascorbic acid, α-tocopherol and BHT were used as the standard compounds. Additionally, some phenolic compounds that responsible for antioxidant abilities of EESB and WESB were screened by liquid chromatography-high resolution mass spectrometry (LC-HRMS). At the same concentration, EESB and WESB demonstrated effective antioxidant abilities when compared to standards. In addition, EESB demonstrated IC50 values of 1.946 μg/mL against acetylcholinesterase (AChE), 0.815 μg/mL against α-glycosidase and 0.675 μg/mL against α-amylase enzymes.

Highlights

  • It has been observed that the consumption of antioxidants from natural products such as nutritious herbs, herbs and spices are beneficial for human health and had effective in reducing the reactive oxygen species (ROS) occurrence and oxidative stress-related diseases [64]

  • It was found that the antioxidant activities of EESB and WESB are similar or close to standards

  • It was shown that the antioxidant ability of EESB and WESB enhanced with increasing concentration (10–30 μg/mL)

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Summary

Introduction

Subs) is endemic plant to Eastern Anatolia. Convolvulus species are used as a laxative in Anatolian folk medicine. In some regions of Anatolia, convolvulus leaves are added to soup in kitchens [1]. This plant is a hairy herbaceous perennial that is drifting or climbing. Its leaves and flowers are solitary, axillary or in 2-5-flowered cymes. It usually grows in fallow or cultivated fields, along roadsides, in dry ditches and at 30–1700 m altitude

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