Antidiabetic and Antioxidant Activity of Leaves of Aegiceras corniculatum (L.) Blanco (Black Mangrove): An in vitro and in vivo Study

Abstract

Abstract Purpose: Aegiceras corniculatum (L.) Blanco, commonly known as Black Mangrove, is a species of shrub or tree mangrove with a distributed Indian mangrove forest and traditionally used to treat diabetes and other health ailments. Aim: The objective of this work was to enlighten the function of Indian mangrove variety of A. corniculatum leaves on carbohydrates blemishing reactions through diabetes using in vitro and in vivo methods. Methods: The aqueous leaves extract of A. corniculatum (ALAc) was assessed for DPPH (1, 1-diphenyl-2-picrylhydrazyl) radical scavenging and in vitro antidiabetic action through α-amylase activity assessment. Diabetes was induced in adult rats of the Wistar strain by intraperitoneal injection of streptozotocin (STZ) (45 mg/kg) prior to that, toxicity of 0.5, 1, 2, 3 and 4g/kgbw was also investigated. The experimental rats were administered with aqueous leaf extract of A. corniculatum (100, 200 and 300 mg/kgbw) and glibenclamide (10 mg/kgbw) in STZ-induced hyperglycemia. On completion of the 60-day treatment, a range of carbohydrate metabolizing enzymes (glucokinase, glucose-6-phosphatase and fructose 1, 6 bisphosphatase) and antioxidantssuch as SOD, GPx, CAT, GSH and LPOwere tested including liver of control and STZ diabetic rats. Main Results: ALAc dose-dependently inhibited α-amylase enzyme and altered the activities of glucose-6 phosphatase, fructose 1, 6-bisphosphatase and liver glucokinaseto very close to control groups as well in antioxidants activities through the oral administration of extract on 300 mg/kgbw. Conclusions: ALAc at doses of 300 mg/kg/day for 60 days provided a significant decrease in diabetic complications and metabolic impairment. Keywords: Aegiceras corniculatum (L.) Blanco, Black Mangrove, Khalsi, Antidiabetic, Streptozotocin (STZ) Cite this Article Geegi PG, Manoharan N. Antidiabetic and Antioxidant Activity of Leaves of Aegiceras corniculatum (L.) Blanco (Black Mangrove): An in vitro and in vivo Study. Research & Reviews: Journal of Herbal Science . 2018; 7(1): 23–31p.