Anti-diabetic activity of different oils through their effect on arylsulfatases

Rima Samarji,Mahmoud Balbaa

doi:10.1186/s40200-014-0116-z

Rima Samarji, Mahmoud Balbaa

Open Access

https://doi.org/10.1186/s40200-014-0116-z

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Abstract

BackgroundDiabetes mellitus (DM) is characterized by the overproduction of the reactive oxygen species which affects the integrity of the lysosomal membrane affecting lysosomal enzymes. The effect of these species is blocked by some natural products as antioxidants. In the current study, groups of normal and streptozotocin (STZ)-induced diabetic rats were treated by Nigella sativa (NS), olive and canola oils and subjected to the study of arylsulfatases as a model of lysosomal enzymes. The aim of the present study is to investigate the effect of STZ-induced diabetes on arylsulfatases in presence and absence of NS, olive and canola oils.MethodsDifferent groups of rats were induced by STZ, treated with different oils and compared to their corresponding control group. All groups were subjected for the assays of blood glucose, insulin, catalase and arylsulfatases. A comparative kinetic study of arylsulfatses was performed to detect the alteration of catalytic characterization.ResultsThe results demonstrated that diabetes causes a significant elevation in the level of hepatic arylsulfatase B and a significant reduction of hepatic catalase as an antioxidant enzyme. NS and olive oils returned catalase and arylsulfatase B activities back near to normal by fixing their catalytic properties. Furthermore, the maximum velocity of arylsulfatases A and B was significantly elevated in the induced diabetes, whereas their Km values were significantly changed. The treatment of diabetic rats by NS and olive oils reduced the degree of significance.ConclusionDiabetes induces significant alterations of the catalytic characters of arylsulfatases and some oils decrease this alteration through an antioxidant-mediated effect.

Highlights

Diabetes mellitus (DM) is characterized by the overproduction of the reactive oxygen species which affects the integrity of the lysosomal membrane affecting lysosomal enzymes
Chemicals and oils STZ, DE-52, insulin ELISA kit and p-nitrocatechol sulfate (p-NCS) salt were purchased from Sigma-Aldrich (Beirut office Lebanon). p-NCS was prepared as a substrate reagent for Arylsulfatase A (ASA) Arylsulfatase B (ASB) as previously explained [28]
STZ injection induces diabetes leading to an increase in Blood glucose level (BGL) more than 120 mg/dl

Summary

Introduction

Diabetes mellitus (DM) is characterized by the overproduction of the reactive oxygen species which affects the integrity of the lysosomal membrane affecting lysosomal enzymes. The effect of these species is blocked by some natural products as antioxidants. The aim of the present study is to investigate the effect of STZ-induced diabetes on arylsulfatases in presence and absence of NS, olive and canola oils. The present research is conducted to investigate the effect of STZ-induced diabetes on arylsulfatases in presence and absence of NS, olive and canola oils. The enzymatic activity of arylsulfatases is assayed in both liver and serum in all groups and the catalytic parameters of ASA and ASB were determined to detect any induced catalytic change of these enzymes in diabetes

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