Antidiabetic activity of Ayurvedic polyherbal formulations Avipattikara Churna and Triphla Churna in streptozotocin and nicotinamide induced diabetic wistar rats

Abstract

Objective : The aim of the present study is to formulate a polyherbal formulation whose formula is given in AFI and evaluate its antidiabetic potential in animals. Method: The polyherbal formulation was prepared using the methanol extracts and aqueous extracts of the given parts of herbs in AFI. The quality of the finished product was evaluated as per the World Health Organization’s guidelines for the quality control of herbal materials. The quality testing parameters of the polyherbal formulation were within the limits. Results : The acute toxicity studies of the polyherbal formulation did not show any toxic symptoms in doses up to 2000 mg/kg over 14 days. The extracts of both the formulations were evaluated for In-vitro and In-vivo anti-diabetic study. The oral anti-diabetic activity of the polyherbal formulation (200 and 400 mg/kg, p.o) was screened against intraperitoneal (i.p.) STZ (35 mg/kg) 15 min after i.p. administration of nicotinamide (200 mg/kg) continuously for 2 days, to induced diabetes mellitus in Wistar rats. The investigational drug was administered for 28 consecutive days, and the effect of the polyherbal formulation on blood glucose levels was studied at regular intervals.