Abstract
Purpose : To determine the anti-diabetic activity of an aqueous extract of Fructus Ligustri Lucidi (FLL) in a rat model of type 2 diabetes (T2D). Method : Body weight (BW), food intake (FI), fasting blood glucose (FBG), glucose tolerance testing and insulin tolerance testing were used to determine the anti-diabetic activity of an aqueous extract of Fructus Ligustri Lucidi (FLL) in a rat model of streptozotocin-induced T2D. Anti-oxidant activity and oxidative stress were assessed by superoxide dismutase (SOD) and malondialdehyde (MDA) analyses, respectively. Results : Following FLL extract treatment, diabetic rat BW increased, while FI and FBG levels decreased. FLL extract increased glucose tolerance and decreased insulin tolerance. Following treatment with 300 and 600 mg/kg FLL extract, MDA levels reached 13.5 ± 0.9 nmol/ml and 13.8 ± 1.1 nmol/ml, respectively. Compared to MDA levels of 19.5 ± 1.1 nmol/ml in diabetic control group, MDA levels was decreased by 30.8 % and 29.0 % after the treatment with 300 and 600 mg/kg FLL extract, respectively, indicating alleviated oxidative stress. Conclusion : The results show that aqueous FLL extract has the potential to alleviate T2D, resulting in reduced FI and FBG, and increased BW. The anti-diabetic activity of FLL extract on T2D may be relevant to reduced oxidative stress burden. Keywords : Fructus Ligustri Lucidi, Type 2 diabetes, Anti-oxidant activity, Glucose tolerance, Insulin tolerance, Anxiety-like behaviour
Highlights
Fructus Ligustri Lucidi (FLL) is widely used as traditional Chinese medicine for strengthening bones and treating kidney and liver diseases [1]
Continuous 35day treatment with FLL extract resulted in an obvious decrease in diabetic rat fasting blood glucose (FBG)
We examined the acute toxicity of FLL extracts and their effects on FBG, Body weight (BW), food intake (FI), Glucose tolerance testing (GTT), Insulin tolerance testing (ITT), anxiety-like behaviour, and oxidative stress were analysed in this study
Summary
Fructus Ligustri Lucidi (FLL) is widely used as traditional Chinese medicine for strengthening bones and treating kidney and liver diseases [1]. FLL was purchased from the Second Affiliated Hospital of Shanxi University of Traditional Chinese Medicine and dried at 50 °C for 48 h. It was ground into powder and extracted in water for 2 days using a Soxhlet extractor. The study was approved by Animal Ethic Committee of the Affiliated Hospital of Shanxi University of Traditional Chinese Medicine (No EA_20160107), and the experiments with rats were in full compliance with the European Communities Council Directive of 24 November 1986 (86/609/EEC) [9]and with the Guidelines laid down by the NIH in the US [10]. The rats were treated with saline or FLL extracts 1 h prior to glucose administration. The rats received saline or FLL extracts 1 h prior to insulin administration. Results are presented as mean ± standard deviation. t-Tests were used to compare differences between two groups, and p < 0.05 was considered statistical significant
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