Abstract

Purpose : To determine the anti-diabetic activity of an aqueous extract of Fructus Ligustri Lucidi (FLL) in a rat model of type 2 diabetes (T2D). Method : Body weight (BW), food intake (FI), fasting blood glucose (FBG), glucose tolerance testing and insulin tolerance testing were used to determine the anti-diabetic activity of an aqueous extract of Fructus Ligustri Lucidi (FLL) in a rat model of streptozotocin-induced T2D. Anti-oxidant activity and oxidative stress were assessed by superoxide dismutase (SOD) and malondialdehyde (MDA) analyses, respectively. Results : Following FLL extract treatment, diabetic rat BW increased, while FI and FBG levels decreased. FLL extract increased glucose tolerance and decreased insulin tolerance. Following treatment with 300 and 600 mg/kg FLL extract, MDA levels reached 13.5 ± 0.9 nmol/ml and 13.8 ± 1.1 nmol/ml, respectively. Compared to MDA levels of 19.5 ± 1.1 nmol/ml in diabetic control group, MDA levels was decreased by 30.8 % and 29.0 % after the treatment with 300 and 600 mg/kg FLL extract, respectively, indicating alleviated oxidative stress. Conclusion : The results show that aqueous FLL extract has the potential to alleviate T2D, resulting in reduced FI and FBG, and increased BW. The anti-diabetic activity of FLL extract on T2D may be relevant to reduced oxidative stress burden. Keywords : Fructus Ligustri Lucidi, Type 2 diabetes, Anti-oxidant activity, Glucose tolerance, Insulin tolerance, Anxiety-like behaviour

Highlights

  • Fructus Ligustri Lucidi (FLL) is widely used as traditional Chinese medicine for strengthening bones and treating kidney and liver diseases [1]

  • Continuous 35day treatment with FLL extract resulted in an obvious decrease in diabetic rat fasting blood glucose (FBG)

  • We examined the acute toxicity of FLL extracts and their effects on FBG, Body weight (BW), food intake (FI), Glucose tolerance testing (GTT), Insulin tolerance testing (ITT), anxiety-like behaviour, and oxidative stress were analysed in this study

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Summary

INTRODUCTION

Fructus Ligustri Lucidi (FLL) is widely used as traditional Chinese medicine for strengthening bones and treating kidney and liver diseases [1]. FLL was purchased from the Second Affiliated Hospital of Shanxi University of Traditional Chinese Medicine and dried at 50 °C for 48 h. It was ground into powder and extracted in water for 2 days using a Soxhlet extractor. The study was approved by Animal Ethic Committee of the Affiliated Hospital of Shanxi University of Traditional Chinese Medicine (No EA_20160107), and the experiments with rats were in full compliance with the European Communities Council Directive of 24 November 1986 (86/609/EEC) [9]and with the Guidelines laid down by the NIH in the US [10]. The rats were treated with saline or FLL extracts 1 h prior to glucose administration. The rats received saline or FLL extracts 1 h prior to insulin administration. Results are presented as mean ± standard deviation. t-Tests were used to compare differences between two groups, and p < 0.05 was considered statistical significant

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Conflict of Interest
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