Antidiabetic activity and chemical characterization of aqueous/ethanol prop roots extracts of Pandanus fascicularis Lam in streptozotocin-induced diabetic rats

Abstract

ObjectiveTo evaluate antidiabetic activity and chemical characterization of aqueous and ethanolic extracts of prop roots of Pandanus fascicularis Lam (P. fascicularis) in streptozotocin (STZ) induced diabetic rats. MethodsEthanol and aqueous extracts were prepared from P. facicularis by percolation and cold maturation, respectively. Anti diabetic activity of prop roots of P. fascicularis at the dose of 250 mg/kg in STZ (i.p / 60mg/kg body weight) induced diabetic rats. The fasting blood glucose levels were measured at 0, 1, 2 and 3 hrs after the treatment of ethanol and aqueous extracts. The ethanol fraction was then subjected to chromatographic analysis and a compound has been isolated and characterized by IR, 1H-NMR and Mass Spectroscopy. ResultsThe reduced blood glucose level was significant (P < 0.001) in the dose of 250 mg/kg of ethanol and aqueous extracts of P. fascicularis, when compared with control. The blood glucose level of diabetic control animals after 3 h was (226.00 ± 1.78) mg/kg, whereas it were (102.00 ±1.73) mg/kg and (131.00 ± 1.92) mg/kg for the groups treated with aqueous extract and ethanol extract, respectively. The ethanol fraction was then subjected to chromatographic analysis and a compound has been isolated. The structure of the isolated compound is may be characterized as Hepta deca-5-ene-1-ol by analysis it's IR, 1H NMR and Mass spectroscopy data. ConclusionThese results demonstrated that aqueous/ethanol extracts of prop roots of P. fascicularis showed significant anti-diabetic activity, consequently this plant might be of value in diabetes treatment.