Abstract
Many medicinal plants are widely grown in South- and Southeast Asia countries. Some of them are traditionally used for treatment of diabetes mellitus such as Andrographispaniculata and Centellaasiatica. In the study, we provided fractionated-extracts of A. paniculata and C. asiatica to increase the concentration of their active compounds and eliminate unexpected substances. The aim of the study was to evaluate the antidiabetes effect of the combination of their fractionated-extracts in male neonatal streptozotocin (STZ)-induced diabetic rats. In the study, diabetes was injected intraperitoneally 90mg.kg-1 BWSTZ to two day-old rats. At the age of three months, the rats were administered with the combination of both fractionated-extracts for 14 consecutive days. We evaluated antidiabetes with parameters of blood glucose levels, morphology of pancreatic islet, β-cell density as well as immunohistochemical pancreatic insulin. The levels of MDA, SOD and GPx were also determined about oxidative stress change after treatment with the combination. In the study, the combination succeeded to lower the blood glucose level in neonatal STZ-induced diabetic rats. Inline with fact, improvement of rat pancreatic islets and β cells density, as well as moderate restoration of pancreatic insulin, were observed after treatment with the combination. The substance could decrease the level of MDA, and increase the levels of SOD and GPx. In conclusion, the combination of fractionated-extracts of A. paniculata and C. asiatica exhibited potential antidiabetic effect to its antioxidative effect in male neonatal STZ-induced diabetes rats.
Highlights
Diabetes mellitus (DM) is chronic diseases induced by damage/disorder in carbohydrate metabolism that resulting hyperglycemic
Measurement of blood glucose level was based on enzymatic colouri metric method using glucose GOD-PAP reagent purchased from DiaSys, Diagnostic Systems GmbH, Holzheim, Germany
Quantitative analysis was demonstrated by TLC densitometry method
Summary
Diabetes mellitus (DM) is chronic diseases induced by damage/disorder in carbohydrate metabolism that resulting hyperglycemic. The incidence of diabetes mellitus every year is always increasing, especially in developing countries. Based on WHO data, in 2012 about 1.5 million deaths were caused by diabetes mellitus (WHO, 2016). In the condition of diabetes mellitus, the decrease of pancreatic β cells can be contributed by apoptosis (Butler et al, 2003), resulting in insulin deficiency and turn increasing blood glucose levels. South- and Southeast Asia countries have big biodiversity togeteher with medicinal plants. Exploration of medicinal plants is very interesting and widely done to provide alternative medicines. A. paniculata and C. asiatica are two examples of traditionally medicine that used for treating
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