Antidepressant activity of Kampavatari rasa, an Herbo-mineral preparation in swiss albino mice

Abstract

Objective: Depression is a characterized by low mood and lack of activity that can implicit on person's thoughts, behavior, feelings, and sense of well-being. Materials and Methods: Equal number (n=6) Swiss albino mice weighing 20-25gm were randomly divided into 4 groups having 6 animals in each group as Control (1% Gum acacia), Standard (Imipramine-10mg/kg), Kampavatari Ras-Plain (KVR-P) (200 mg/kg) and Kampavatari Ras-Gold (KVR-G) (200 mg/kg). Each mouse was administered with respective vehicle / drug one hour prior to experiment. First two minute reading was not recorded but last four minutes activity was noted for mobility and immobility both in forced swim and tail suspension test. Statistical Analysis: Observations are mean ± S.E.M. One- way ANOVA test followed by Post-hoc Dunett’s Multiple comparison. p Results: Mean duration of immobility for the control group was observed to be 141.33±5.5 sec and 164.16±10.54sec respectively for forced swim test (FST) and tail suspension test (TST). The standard (Imipramine, 10mg/kg) showed was 211.83±48sec and 212.5±6.80 respectively for forced swim test (FST) and tail suspension test (TST). Kampavatari Ras-Plain (KVR-P) at the dose of 200 mg/kg showed immobility time of 189±48sec and 203.55±6.02 for forced swim test (FST) and tail suspension test (TST) respectively. Similarly, Kampavatari Ras-Gold (KVR-G) at the dose of 200 mg/kg showed immobility time of 210±9.8sec and 193.33±6.7sec for forced swim test (FST) and tail suspension test respectively. Conclusion: Kampavatari Ras-Plain and Kampavatari Ras-Gold at the dose of 200mg/kg has shown antidepressant effect when compared to control which is comparable to standard Imipramine in Swiss albino mice. Keywords: Anti-depressant effect, Forced swim test (FST), Tail suspension test (TST), Kampavatari Ras-Plain, Kampavatari Ras-Gold.