Antidepressant Activity and Identification of Chemical Compounds Extract Mustard Leaves (Brassica juncea L.)

Abstract

Depression is a common disease that affects more than 300 million people of all ages which is generally accompanied by insomnia. In 2030 this disease is thought to be the biggest contributor to the burden of the disease worldwide. Mustard is one of the vegetables produced in Southeast Sulawesi, especially in the Wua-wua area, Kendari, where its use as a medicine is still lacking. This study aims to test the antidepressant activity of mustard leaf extract, and identify the class of extract chemical compounds. The long-term goal of this research is the manufacture of herbal preparations for either herbal or standardized herbs for the treatment of depression. Mustard leaf extract obtained from maceration contains flavonoids, saponins, sterols/triterpenoids, and terpenes. The testing of activities with the Open field test method, Forced swim test, and Tail suspension test of mice were carried out at several test doses (100 mg/kg bw, 200 mg/kg bw, and 400 mg/kg bw). The test results obtained was analyzed by One-way Analysis of Variance (ANOVA) (SPSS 16.0 program) indicating that the antidepressant effect shown in the extract dose of 100 mg/kg bw and 200 mg/kg bw.