Anticonvulsant like Activity of Silybin Correlates Blood Glucose Levels in PTZ induced Chemo-convulsive Rats

Abstract

The objective of this study is to observe the impact of Silybin (SIB) on the link between blood glucose and seizure prevention in chemo-convulsive rats. To produce seizures, adult male rats were administered with pentylenetetrazol (PTZ-105 mg/kg i.p.) at the CD97 dose. SIB (100 mg/kg.p.o.) was administered for two weeks. The animals were subjected to chemo convulsions on the 14th day, and the effectiveness of SIB to reduce clonus-type chemo convulsions (CC) was recorded, as well as blood glucose levels measured with a one-touch glucometer.Morphometric analysis and chimney tests were evaluated to know the neurotoxic profile of SIB, while actophotometer, rota rod, and hole board tests were used for behavioural analysis.Pre-treatment with SIB (100 mg/kg p.o) resulted in a significant delay in the onset of CC (*p<0.05) and a significant reduction in the duration of CC (**p<0.01) as compared to controls. The acute dose of SIB significantly reduced blood glucose levels. In addition, acute administration of SIB considerably lowered blood glucose levels and promoted body weight of animals whereas chimney tests, indicating that, non-neurotoxic profile of silybin to animals. Additionally, behavioural tests revealed that no significant alterations in muscular coordination, locomotion, or anxious behaviour were noticed in animals. In summary; results reveal that the NDH + PTZ groups had considerably greater hyperglycemia levels, which is reflected in the early onset and longer duration of chemo convulsions in rat. SIB treatment, However, there was a significant reduction in elevated glucose levels, as well as a considerable delay in the onset and duration of seizures, demonstrating the relationship between blood glucose and Anticonvulsant like activity against chemo convulsive episodes. Keywords: Silybin, Hyperglycaemia, Pentylenetetrazol, chemo convulsions, seizure protection