Abstract
ConclusionThe probit transformation for the plotting of immune hemolysis produced by measured amounts of fresh human serum was found to be suitable for titration of complement. Serum from a patient with a high level of C′ activity was found to become highly anticomplementary when heated to 56°C for 30 minutes, but inactivation by heating for 30 minutes at 52°C or below produced little anticomplementary activity in this serum. Addition of an equal quantity of 9% bovine serum albumin prior to heating also prevented the expression of the anticomplementary activity. Ability of serum to become highly anticomplementary as a result of heating is not necessarily associated with diminished activity of its endogenous complement. Conversely, sera with low levels of C′ may not become anticomplementary as a result of heating.
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