ANTICOAGULANT EFFECT OF CHARONINSULFURIC ACID

Abstract

The anticoagulant effect of charoninsulfuric acid, a polysaccharide sulfuric acid obtained from the mucus of [Charonia lampas] was investigated both in vivo and in vitro. The results obtained were as follows:1) Charoninsulfuric acid is a strong anticoagulant. The clotting time of whole blood was prolonged slightly by adding only 1γ charoninsulfuric acid to 1ml. of rabbit blood or 0.1γ to 1ml. of human blood. The more its concentration increased, the longer the clotting time became, and in concentration of over 50γ in 1ml. of rabbit or human blood the clot was not detected over 24 hours.when charoninsulfuric acid was intravenously injected (1 mg per Kg of body weight), the clotting time of rabbit whole blood was prolonged and reached the maximal level after 15 minutes. Then, the clotting time decreased gradually to normal level within 1 hour or so.2) In experiments in vitro, charoninsulfuric acid produced no remarkable prolongation of prothrombin time of rabbit plasma by adding charoninsulfuric acid up to 20γ per ml. of blood, while prothrombin time of human plasma was prolonged by adding only 0.05γ per ml. of blood and the more the concentration increased, the more the prothrombin time was prolonged.Prothrombin time of rabbit plasma measured before and after the intravenous injection of charoninsulfuric acid (1mg. per Kg. of body weight) revealed no remarkable change.3) Stable factor (SPCA) was not affected by charoninsulfuric acid both in vitro and in vivo.4) Charoninsulfuric acid has not only anti-labile factor activity but also anti-prothrombin activity.5) Antithromboplastic activity of charoninsulfuric acid was demonstrated by thromboplastin generation test both of Matsuoka-Yamanaka and of Biggs-Douglas.6) Thromboplastin dilution curve of rabbit plasma, which means the prothrombin time measured with serial twofold diluted rabbit brain extract, was also prolonged after the intravenous injection of charoninsulfuric acid (1mg. per Kg. of body weight).7) When charoninsulfuric acid was intravenously injected (1mg. per Kg. of body weight), leucocyte and platelet count of rabbit were temporarily decreased, while the erythrocyte count showed no remarkable change.