Anticoagulant activity of a sulfated Lachnum polysaccharide in mice with a state of hypercoagulability

Abstract

A sulfated polysaccharide, designated as SLEP-1, was obtained after sulfation of the exopolysaccharide (LEP-1) which was isolated from Lachnum. The degree of substitution (DS) of sulfate group of SLEP-1 was 1.97. SEM images of SLEP-1 revealed laminated structure in mesh. UV, FT-IR and 13C NMR spectra indicated that the LEP-1 was sulfated successfully. The result of the anticoagulant activity in vitro showed that both of LEP-1 and SLEP-1 could effectively prolong activated partial thromboplastin time (APTT) and thrombin time (TT) of the normal mice plasma, in which SLEP-1 was more effectively than those of LEP-1, and dose–effect relationships were found. According to the bleeding time (BT), clotting time (CT), APTT, PT, prothrombin time (TT), fibrinogen (FIB), AT-III activity and FXa concentration of the hypercoagulable mice, it indicated that SLEP-1 (30mg·kg−1 and 90mg·kg−1) had strong inhibitory effect on intrinsic coagulation pathway, which could also enhance fibrinolytic activity. It may constitute an anticoagulant drug of interest in anticoagulant therapy.