Anti-cancer potential of methyl gallate isolated from Bambangan (Mangifera pajang) in MCF-7 cell line

Abstract

Event Abstract Back to Event Anti-cancer potential of methyl gallate isolated from Bambangan (Mangifera pajang) in MCF-7 cell line Azlen Che Rahim1, Mohd Fadzelly Abu Bakar1*, Nur Kartinee Kassim2, Johnson Stanslas2 and Wan Nor I’zzah Wan Mohamad Zain3 1 Universiti Tun Hussein Onn Malaysia, Malaysia 2 Putra Malaysia University, Malaysia 3 Universiti Teknologi MARA, Malaysia Background Mangifera pajang or widely known as Bambangan is an indigenous fruit of Borneo Island. The kernel part of this fruit has been reported to display anti-cancer potential. This study was conducted to isolate the major bioactive compound from the kernel of Bambangan and determine the cytotoxic activity and apoptosis-inducing ability of the compound in MCF-7 cell line. Methods Methyl gallate (C8H8O5) has been successfully isolated from the methanol crude extract of Bambangan kernal. Identification of methyl gallate was done using spectroscopic method analysis such as infrared (IR), mass spectrometry (MS), nuclear magnetic resonance (NMR) and compared to reported data. Cytotoxic activity of methyl gallate was determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The effect of methyl gallate on cell cycle and apoptosis induction was assessed using flow cytometry analysis. Results The results showed that methyl gallate induced strong cytotoxic activity in MCF-7 cell line with IC50 value of 54.7 µM. Methyl gallate increased cell population in sub-G1 phase indicating apoptosis and cell cycle arrest at G0/G1. In addition, staining with Annexin V-FITC and propidium iodide revealed that total apoptotic cells were increased after incubation with the compound in a time dependent manner. Conclusion As a conclusion, methyl gallate isolated from Bambangan induced anti-cancer potential in breast cancer cell. Study on the mechanism of action as well as the efficacy of this compound in in vivo is suggested moving forward Acknowledgements This study was funded under Fundamental Research Grant Scheme (Vot: 1560) as well as UTHM contract grant (H277). Special thanks to Universiti Tun Hussein Onn Malaysia (UTHM), Universiti Putra Malaysia (UPM) and Universiti Teknologi MARA (UiTM) for the use of laboratories and technical assistance. Keywords: Mangifera pajang, Methyl gallate, MCF-7, Apoptosis, cytotoxic Conference: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”, Putrajaya, Malaysia, 3 Dec - 5 Feb, 2019. Presentation Type: Poster Presentation Topic: Cancer Citation: Che Rahim A, Abu Bakar M, Kassim N, Stanslas J and Wan Mohamad Zain W (2019). Anti-cancer potential of methyl gallate isolated from Bambangan (Mangifera pajang) in MCF-7 cell line. Front. Pharmacol. Conference Abstract: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”. doi: 10.3389/conf.fphar.2018.63.00149 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 16 Oct 2018; Published Online: 17 Jan 2019. * Correspondence: Dr. Mohd Fadzelly Abu Bakar, Universiti Tun Hussein Onn Malaysia, Parit Raja, Malaysia, fadzelly@uthm.edu.my Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Azlen Che Rahim Mohd Fadzelly Abu Bakar Nur Kartinee Kassim Johnson Stanslas Wan Nor I’zzah Wan Mohamad Zain Google Azlen Che Rahim Mohd Fadzelly Abu Bakar Nur Kartinee Kassim Johnson Stanslas Wan Nor I’zzah Wan Mohamad Zain Google Scholar Azlen Che Rahim Mohd Fadzelly Abu Bakar Nur Kartinee Kassim Johnson Stanslas Wan Nor I’zzah Wan Mohamad Zain PubMed Azlen Che Rahim Mohd Fadzelly Abu Bakar Nur Kartinee Kassim Johnson Stanslas Wan Nor I’zzah Wan Mohamad Zain Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.