Abstract
This study reports the synthesis of silver nanoparticles using amino acid L-histidine as a reducing and capping agent as an eco-friendly approach. Fabricated L-histidine-capped silver nanoparticles (L-HAgNPs) were characterized by spectroscopic and microscopic studies. Spherical shaped L-HAgNPs were synthesized with a particle size of 47.43 ± 19.83 nm and zeta potential of −20.5 ± 0.95 mV. Results of the anticancer potential of L-HAgNPs showed antiproliferative effect against SiHa cells in a dose-dependent manner with an IC50 value of 18.25 ± 0.36 µg/mL. Fluorescent microscopic analysis revealed L-HAgNPs induced reactive oxygen species (ROS) mediated mitochondrial dysfunction, leading to activation of apoptotic pathway and DNA damage eventually causing cell death. To conclude, L-HAgNPs can act as promising candidates for cervical cancer therapy.
Highlights
Nanotechnology is the engineering of functional systems at the molecular scale
This study focused on an eco-friendly approach for the synthesis of L-histidine-capped silver nanoparticles (L-HAgNPs)
Reduction of Ag (NO3 )2 by L-histidine was identified by the color transition from colorless to brown color, which was further confirmed by UV–Visible spectral analysis (Figure 1I)
Summary
Nanotechnology is the engineering of functional systems at the molecular scale. Metal nanoparticles have become powerful materials in biomedicine [1,2,3]. Unique physicochemical properties of metal nanoparticles have increased the attention of researchers in recent decades in various fields such as catalysis and optoelectronics [4]. Silver nanoparticles (AgNPs) have received much attention in the field of biomedicine due to their wide pharmacological properties such as antibacterial, antifungal, anti-inflammatory, antiviral activities, and biosensing properties [5,6,7,8,9]. Silver nanoparticles were synthesized through physical and chemical methods, which suffered certain limitations provoking the researchers toward biogenic sources 4.0/).
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