Abstract
BackgroundColorectal cancer (CRC), with a growing incidence trend worldwide, is resistant to apoptosis and has uncontrolled proliferation. It is recently reported that probiotic microorganisms exert anticancer effects. The genus Bifidobacterium, one of the dominant bacterial populations in the gastrointestinal tract, has received increasing attention because of widespread interest in using it as health-promoting microorganisms. Therefore, the present study aimed to assess the apoptotic effects of some bifidobacteria species on colon cancer cell lines.Methods The cytotoxicity evaluations performed using MTT assay and FACS-flow cytometry tests. Also, the effects of five species of bifidobacteria secretion metabolites on the expression level of anti- or pro-apoptotic genes including BAD, Bcl-2, Caspase-3, Caspase-8, Caspase-9, and Fas-R studied by real-time polymerase chain reaction (RT-PCR) method.ResultsThe cell-free supernatant of all studied bifidobacteria significantly decreased the survival rates of colon cancer cells compared with control groups. Flow cytometric and RT-PCR results indicated that apoptosis is induced by bifidobacteria secretion metabolites and the mechanism for the action of bifidobacteria species in CRC prevention could be down-regulation and up-regulation of anti-apoptotic and, pro-apoptotic genes.ConclusionsIn the present study, different bifidobacteria species showed anticancer activity on colorectal cancer cells through down-regulation and up-regulation of anti-apoptotic and pro-apoptotic genes. However, further studies are required to clarify the exact mechanism of apoptosis induction by bifidobacteria species.
Highlights
Cancer as a major public health problem has a growing incidence trend worldwide
Some studies proposed that the ingestion of certain microorganisms decreases both the risk of developing certain types of cancer and tumor growth, so a lot of attention has been focused on probiotic yeasts and bacteria like lactobacilli and bifidobacteria [7,8,9,10,11]
The Half-maximal inhibitory concentration (IC50) after treatment by prepared cell-free supernatants of bifidobacteria were determined a range between 65 μg/mL to 80 μg/mL for HT-29 and Caco-2 cells at 48 h and the control group (KDR/293 cells) were treated with the highest determined concentration (80 μg/mL)
Summary
Cancer as a major public health problem has a growing incidence trend worldwide. CRC, the most common malignancy of the gastrointestinal tract, is the third and second most common cancer in males and females, respectively, and is the fourth leading cause of cancer death worldwide [3, 4]. Some studies proposed that the ingestion of certain microorganisms decreases both the risk of developing certain types of cancer and tumor growth, so a lot of attention has been focused on probiotic yeasts and bacteria like lactobacilli and bifidobacteria [7,8,9,10,11]. The genus Bifidobacterium, one of the dominant bacterial populations in the gastrointestinal tract, has received increasing attention because of widespread interest in using it as health-promoting microorganisms. The present study aimed to assess the apoptotic effects of some bifidobacteria species on colon cancer cell lines
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
