Anticancer Effect of Alstonia Scholaris Against the Methyl-Methanesulphonate-Induced Genotoxicity in Human Peripheral Lymphocytes in Vitro

Abstract

Abstract: Alstonia scholaris is used in various Ayurvedic preparations like Saptaparnasatvadi vati, Saptachadadi vati, Saptachhadadi vati, Saptacchadadi taila, Saptacchadadi kvatha and Saptaparna ghanasara or uses of Alstonia scholaris mainly in Whooping cough, Malaria, Jaundice, Gastric complaint, Headache, Asthma, Stomachache, Wound, Fever and also much effective in all types of cancer. The antigenotoxic potential of Alstonia scholaris extract (AS) was demonstrated on the methyl methanesulphonate (MMS) induced genotoxicity. In vitro studies were carried on human lymphocyte culture. We have used chromosomal aberration (CA), sister chromatid exchange (SCE) and cell cycle kinetics (CCK) with and without S9 mix. as markers in this experiments. Four doses viz., 100, 150, 200, 250 μl/ml per culture were selected and found that Alstonia scholaris extract significantly reduces the frequencies of chromosomal aberration, sister chromatid exchanges and enhances RI in vitro .It was also noticed that the antigenotoxic potential of AS shows dose – response relationship. The results suggest that AS was a potent anticarcinogen may contribute to the cancer prevention.