Anticancer Effect in Human Glioblastoma and Antioxidant Activity of Petroselinum crispum L. Methanol Extract

Abstract

Parsley (Petroselinum crispum L.) has been used as food, spices and in folkloric medicine. Several scientific researches have been focalized on anti-inflammatory, anti-proliferative, antioxidant and other pharmacological activities of parsley. The aim of the present study was to evaluate the phytochemical composition, antioxidant and anticancer activity of P. crispum L aqueous and methanol extracts against Human glioblastoma cells U87MG. Adhesion assay was realized on different protein matrices (fibrinogen, fibronectin and poly-L-lysine) and the anti-proliferative effect was performed. Compared to aqueous extract, the methanol extract presented an important level of phenol contents. Five phenolic compounds were found using HPLC-DAD with quinic acid as the most abounded followed by gallic acid, acacetin, protocatechuic acid and Cirsilineol with 120753.07 ± 27450; 190 ± 25; 53.83 ± 10; 13.7 ± 2.5 and 2 ± 0.3 µg/mL respectively. The DPPH, ABTS+, OH radical, Iron (II) chelation and FRAP assays exhibited that methanol extract show a modulate antioxidant activity. The methanol extract shows the highest ability to inhibit cell adhesion to different protein matrices. In addition, it was found as a potential anti-proliferative. These results suggest for the first time that P. crispum methanol extract presents anti-adhesion and anti-proliferative proprieties.