Anticancer, Antioxidant, Antimicrobial and Cytogenetic Effects of Ethanol Leaves Extract of Carthamus tinctorius

Abstract

Total polyphenol contents (TPCs), Total flavonoids contents (TFCs) and antioxidant activity (AA)% of ethanol leaves extract (ELEs) of Carthamus tinctorius was investigated, represented by the detection of the antibacterial activity against some pathogenic microbes, as well as the cytotoxicity assay (MTT) and programmed cell death (Apoptosis) by DAPI assay was studied on MCF-7 cell line. Regarding the activity against bacteria, the results showed that the greatest effect of the (ELEs) was on the growth of Gram+ve Staphylococcus aureus bacteria with a diameter of the inhibition zone reached (31 mm), followed by Gram-ve bacteria Pseudomonas aeruginosa and Gram-ve E.coli with an inhibition zone diameter reaching to (22.66 and 22.33 mm) respectively, The lowest effect recorded on the growth of C. albicans, the diameter of the inhibition zone reached to (21,33 mm). As for the effectiveness against oxidative stress and programmed death, the results showed that the extract had a good effect on cancer cells by causing a shrink and destroy them. All these findings were significantly correlated with the concentration of the total content of phenols and flavonoids in the extract. It was also observed that a clear effects of cytogenetic indicators represented in the drooped in the blastogenic index (BI) and the mitotic index (MI) increased by increasing the concentration. This concentration-dependent cytotoxic action is much like the plant alkaloids in chemotherapies.