Abstract
<p>This study was aimed to evaluate the chemical composition of ethanolic leaf extract of a marine macro-alga <em>Sargassum wightii</em> (ELESW) followed by its vascular endothelial growth factor (VEGF) induced anti-angiogenic effect against human umbilical vein endothelial cells (HUVECs). The effect of ELESW on cell viability and its potential against <em>in vivo</em> angiogenesis was assessed by using the MTT and CAM assay respectively. The phenolic, flavonoid, polysaccharides, and protein content of ELESW were found to be 56.0 ± 4.1 mg GAE/g, 26.3 ± 3.6 mg QE/g, 284.2 ± 6.2 μg/mL, 100.9 ± 8.9 μg/mL, respectively. In angiogenesis, ELESW (100, 500 and 1,000 μg/mL) significantly inhibited VEGF induced angiogenesis as well as VEGF-induced proliferation and tube formation of HUVECs in a dose-dependent manner. Upon stimuli-tion by VEGF, HUVECs rapidly increased reactive oxygen species production, which was significantly blocked by treatment with ELESW. These findings suggesting that <em>S. wightii</em> could be a valuable candidate of therapeutic efficacy.</p>
Highlights
Most often, the osteosarcoma takes place in new developed bones of infants and adolescents accompanied by subsequent respiratory failure and lungs metastases (Marine et al, 2004; Bielack et al, 2002)
in vivo angiogenesis was assessed by using the MTT
which was significantly blocked by treatment with ELESW
Summary
The osteosarcoma takes place in new developed bones of infants and adolescents accompanied by subsequent respiratory failure and lungs metastases (Marine et al, 2004; Bielack et al, 2002). Effec-tive curing strategies and treatments of osteosarcoma include radio surgery, and adjuvant chemotherapeutic agents including ifosfamide, cisplatin, methotrexate, doxorubicin and etoposide (Marine et al, 2004), they have frequently reported to exert adversary side effects. These chemotherapeutic agents have displayed sever toxicity and chemoresistance in this assertive stage of cancer. This has increased a huge demand of exploring more effective and selective types of chemotherapeutic agents with less or no adverse effects to treat osteosarcoma complications (Bielack et al, 2002; Goorin et al, 1991). Since plant-derived phytochemicals are recommended as potential drug targets to treat cancer and angiogenesis-related diseases (Coussens et al, 2002)
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