Abstract
Abstract In the present work, green nanotechnology was used to synthesize silver nanoparticles of different sizes to study their anti-cancer activity against He-La cervical. The synthesis method is easy, simple, environment friendly and does not require any sophisticated labs. This method utilizes principles of green chemistry. Silver nanoparticles were synthesized using electrolytic deposition technique with black tea leaf extract as capping agent. The as-synthesized nanoparticles were characterized using XRD, TEM, and UV-Visible and FTIR spectroscopy techniques. Elemental analysis of the X-ray graphs reveals that highly pure silver nanoparticles are synthesized. Activity of the Ag NPs against cell lines was observed in a dose-dependent manner using MTT assay and it was found that as-synthesized silver nanoparticles inhibited the growth of He-La cervical cancer cell lines almost up to 70-75% respectively. The IC50 value of sample against He-La cervical cancer cell lines were obtained at 30-fold dilutions of concentration of 178 ppm
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