Anticancer activity of gelatin-asparaginase nanobiocomposite against cervical and brain cancer cell lines

Abstract

l-Asparaginase acts as a therapeutic agent for cancer. The free l-asparaginase has innumerable side effects on the human body. The source of l-asparaginase also plays a major role in the effectiveness of the therapeutic agent. In the present research work, l-asparaginase was produced from a fungal strain Aspergillus terreus. The free enzyme was then immobilised onto a nanobiocomposite consisting of Gelatin and β-cyclodextrin. Both Gelatin and β-Cyclodextrin are biodegradable and are non-toxic. The nanobiocomposite was subjected to characterization studies like the UV–Vis spectra analysis which confirms the presence of the nanobiocomposite. The FTIR analysis of the nanobiocomposite confirmed the functional group present in the nanobiocomposite. The NMR analysis confirmed the chemical shift of the nanobiocomposite. The AFM analysis was used to study surface morphology of the nanobiocomposite. The anticancer activity for the brain cancer cell line and cervical cancer cell line was found as 42.13% at 500 μg/ml and 48.60% at 62.5 μg/ml respectively.