Abstract
Sea Cucumber Holothuria atra is one of marine organisms has been used as a new source of novel bioactive compounds. Many of them have been used as the lead compounds in discovery of new anticancer drugs. The objective of this study was to determine the active fractions of sea cucumber (H. atra) which have anticancer activity. H. atra was macerated using ethanol and the extract was freezedried using a freeze dryer. The crude extract was partitioned using n-hexane, ethyl acetate, and methanol-water (3:1:1:1). Cytotoxicity test was performed using HeLa (cervic cancer) cell line and MCF-7 (breast cancer) cell line based on the MTT assay. The crude extract of H. atra showed the best cytotoxic activity against HeLa cells (IC50 = 12.48 µg/mL) and MCF-7 cells (IC50 = 17.90 µg/mL). The toxicity tests showed the IC50 value of the n-hexane fraction, ethyl acetate fraction, and methanol-water fraction against HeLa cells HeLa (IC50 = 76.45 µg/mL; 77.95 µg/mL; 14.27 µg/mL) and MCF-7 cells (IC50 = 58.50 µg/mL; 59.59 µg/mL; 14.33 µg/mL).
Highlights
Teripang merupakan komoditas perikanan yang bernilai ekonomis penting karena kandungan nutrisi yang tinggi, mudah didapatkan dan sejak lama dimanfaatkan sebagai makanan yang dipercaya dapat menyembuhkan beberapa penyakit dan meningkatkan kesehatan
Sea Cucumber Holothuria atra is one of marine organisms has been used as a new source of novel bioactive compounds
Many of them have been used as the lead compounds in discovery of new anticancer drugs
Summary
Nurul Mutia Putram*1, Iriani Setyaningsih, Kustiariyah Tarman, Muhammad Nursid. Cara sitasi: Putram NM, Setyaningsih I, Tarman K, Nursid M. Penelitian ini bertujuan untuk menentukan aktifitas fraksi aktif teripang (H. atra) sebagai antikanker. Ekstrak kasar selanjutnya dipartisi menggunakan pelarut n-heksana, etil asetat, dan metanol-air (3:1:1:1). Uji sitotoksik dilakukan menggunakan sel HeLa (kanker serviks) dan sel MCF-7 (kanker payudara) berdasarkan metode MTT assay. Ekstrak kasar H. atra menunjukkan aktivitas sitotoksik terhadap sel HeLa dengan nilai IC50 = 12,48 μg/mL dan terhadap sel MCF-7 dengan nilai IC50 = 17,90 μg/mL. Uji sitotoksik menunjukkan nilai IC50 fraksi n-heksana, fraksi etil asetat, dan fraksi metanol-air terhadap sel HeLa berturut-turut adalah IC50 = 76,45 μg/mL; 77,95 μg/mL; 14,27μg/mL) dan pada sel MCF-7 berturut-turut adalah IC50 = 58,50 μg/mL; 59,59 μg/mL; 14,33 μg/mL)
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
