Abstract
Adjuvants are essential to boost the immune response to inoculated antigen and play a central role in vaccine development. In this study, we investigated the efficacy of several adjuvants in the production of anti-bovine serum albumin (BSA) antibodies in silver catfish. Two hundred and seventy juvenile silver catfish (60–80 g) of both sexes were intraperitoneally vaccinated with BSA (200 µg/fish) alone or mixed to the following adjuvants: Freund’s complete adjuvant (FCA), Freund’s incomplete adjuvant (FIA), aluminum hydroxide (AlOH), Montanide, four types of cytosine-phosphate-guanine (CpG) oligodeoxynucleotides (ODNs) and three concentrations of β-glucan, and the immune enhancing property was evaluated by measuring anti-BSA antibodies in blood samples at biweekly intervals. Our results demonstrated that CpGs ODNs and β-glucan were as effective as classical adjuvants (FCA, FIA, AlOH and Montanide) in promoting anti-BSA antibodies and that the kinetics of antibody production induced by all adjuvants used in our study had a similar trend to that observed in other fish species, with a peak at 28 days post-vaccination. These results may be useful for the selection of adjuvants for vaccine formulation intended for silver catfish and for the development of vaccine and vaccination strategies to other fish species.
Highlights
Aquaculture is the fastest growing animal protein production industry in the world and, as such, is facing enormous challenges
Knowing that there are no studies on the efficacy of vaccine and adjuvant in silver catfish (Rhamdia quelen), we aimed to evaluate the effectiveness of oil-based adjuvants (FCA, Freund’s incomplete adjuvant (FIA) and Montanide), AlOH and two classes of pathogen-associated molecular patterns (PAMPs) (b-glucan and four different types of CpG ODNs) in potentiating the antibody production to a model antigen in this important South American fish species
We first describe the effectiveness of several types of classical and experimental adjuvants in improving specific antibody production in silver catfish immunized with bovine serum albumin (BSA)
Summary
Aquaculture is the fastest growing animal protein production industry in the world and, as such, is facing enormous challenges. The classical water in oil (w/o) adjuvant, known as Freund’s incomplete adjuvant (FIA), has long been used for immunization studies in mammals and fish [3], and it is capable of inducing a robust humoral immune response that stands as reference when other classes of adjuvant are evaluated. In fish, it is widely acknowledged, that intraperitoneal injection of oil-based adjuvanted vaccine might cause organ adherence and a poor Th1 lymphocyte response that limits its use against intracellular pathogens [4]. Research on novel immunoadjuvants for aquaculture species are in great demand
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Schedule a callMore From: Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas
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