Antibody Response in Domestic Fowl as Affected by Different Immunization Methods

E Petrovský,Jaromíra Kalová,V Benda

doi:10.2754/avb198756040449

Abstract

The differences are described in this paper as observed in the formation of antibodies an adult hens following application of different routes of immunization and using two antigens distinguished by different dependences on the thymus, actually the SRBC and B. abortus. Formation of the agglutination antibodies to SRBC was inferior to the latter, the i.m. immunization method of antigen application with adjuvant, in conjuction with simplicity of the procedure, in general leading to optimum titres of specific antibodies.

Highlights

It was possible to demonstrate that the experimental birds responded better to B. abortus than to SRBC (1.46 and 1.63, respectively)
Selection of convenient immunization scheme, and in particular of the method of antigen administration is an essencial precondition for the study of immune response
The results of our experimentation were in support of this finding, despite the fact that the experimental birds responded with antibody titres that were markedly lower, in all probability due to the different concentration of SRBC and the higher age of chicks

Summary

Ab s t r act

Ben d a: Antibody Response in Domestic Fowl as Affected by Different Immunization Methods. The differences are described in this paper as observed in the formation of antibodies an adult hens following application of different routes of immunization and using two antigens distinguished by different dependences on the thymus, the SRBC and B. abortus. In thi~ sphere, a number of conclusive differences have been observed in chicks (Van der Zijpp 1983) where, besides the individual characters of the receptor, such as its age, sex, and individual degree of immunoreactivity, it is possible to markedly influence the dynamiCS of antibody formation by the method of immunization used. In this paper are described the differences observed following the application of "thymus-dependent" - SRBC) or "thymus-independent" (Brucella abortus) antigens in the experimental material used

Immunization procedure

Results

Injection route Response Interaction Total

Discussion

Ref ere n c e s