Antibody Protein Hemagglutinin Subunit Pili with MW 49,8 kDa Shigella dysenteriae can inhibit Shigella dysenteriae adhesion on Mice Enterocyte

Abstract

–Among four species of Shigella, Shigella dysentriae (S. dysenteriae) is the most virulent species and often isolated from cases of shigellosis. The initial step in the development of colonization by S. dysenteriae is its ability to adhere toward mucosal surface by using adhesion molecule. Adhesion proteins of bacteria found in the pili and cell wall was evident as the hemagglutinin protein (HA). Until now there is no effective vaccine to Shigellosis in accordance with the expectations formalized by the WHO. This study aimed to prove that HA protein subunit pili with MW 49.8 kDa of S. dysenteriae antibody can inhibit the adhesion of S. dysenteriae on enterocyte of mice by using dose response and immunocytochemistry test (IT) . Dose response method with the implementation of dose dilution of 1/400, 1/800, 1/1600, 1/3200, 1/6400, 1/12800 and 0 (control). While IT used with the implementation dose dilution HA protein sub-unit pili MW 49.8 kDa of 1/500, 1/1000, 1/2000, 1/4000, 1/8000, 1/16000 and 0 (control). ANOVA analysis results of dose response method showed that HA protein subunit pili with MW 49.8 kDa S. dysenteriae antibody test with showed a significant difference of the adhesion index among treatment dose (p value = 0.000). It can be seen that lower dose of HA protein subunit pili with MW 49.8 kDa of S. dysenteriae antibody could increase adhesion index. HA protein sub-units pili MW 49.8 kDa Antibody dilution has a close relationship and a significant influence on the adhesion index (r = -0.724, p = 0.000, R square = 0.52, p