Abstract
A study was undertaken of the immune response of 2-year old plaice, maintained in the aquarium at temperatures between 10° and 15°C, to the administration of a heat-killed, sonicated, freeze-dried Vibrio anguillarum vaccine. This was given as a single injection, in saline (6 mg dry weight of bacteria), suspended in Freund's complete adjuvant (4.5 mg dry weight of bacteria), or fed as 1 mg dry weight of bacteria per gram wet weight of diet. Humoral antibody production, both in blood and mucous secretions of the intestine and skin, was measured one year after injection or after a year of daily feeding with the vaccine. Antibody to Vibrio antigens was detectable in higher titres in the intestinal extract from orally immunized plaice, than in their sera. Parenteral immunization with adjuvant resulted in high and persistent antibody titres in the serum, while only small amounts of antibody were found in the intestinal and cutaneous secretions. No circulating antibodies were detectable one year after injection with bacteria in saline. Gel filtration of serum and mucus showed the antibody activity to be associated with a high molecular weight fraction. The production of antibodies in the plaice is discussed, together with the possible efficacy of oral immunization in the prophylaxis of vibriosis.
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