Abstract
DURING the past few years, two new methods have been described for use in investigating in vitro production of antibodies against sheep erythrocytes by isolated cells. In the first method1,2 a suspension of spleen cells is mixed with sheep erythrocytes, embedded in agar or carboxy-methylcellulose and incubated with guinea-pig complement. Under these conditions, plaque formation occurs as a consequence of the production of haemolysins by certain spleen cells. With the second method3,4 a suspension of spleen cells is incubated with sheep red blood cells, but the cells are not embedded in a semi-solid medium. In this environment clusters of agglutinated sheep erythrocytes are formed around a number of spleen cells. In the present investigation the results obtained by these two methods are compared and the observed differences are discussed.
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