Abstract
Aflatoxin B 1 antibody (aAFB 1) covalently attached to cysteamine functionalized-gold nanoparticles (C-AuNP) has been immobilized onto 4-mercaptobenzoic acid (MBA) based self assembled monolayer (SAM) on gold electrode (MBA/Au), for the fabrication of BSA/aAFB 1-C-AuNP/MBA/Au immunoelectrode. This immunoelectrode has been characterized by Fourier Transform Infrared Spectroscopy (FT-IR), Scanning Electron Microscopy (SEM) and electrochemical characterization techniques. The electrochemical response studies reveal that the BSA/aAFB 1-C-AuNP/MBA/Au immunoelectrode can be used to detect AFB 1 in the range of 10–100 ng dL − 1 and has sensitivity as 0.45 μA ng − 1 dL, limit of detection as 17.90 ng dL − 1 and a response time of 60 s.
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