Abstract
HLA specificity of alloantibodies involved in antibody-dependent cell-mediated cytotoxicity (ADCC) was investigated using PHA-induced lymphoblasts ( PLB ) from peripheral blood and Epstein-Barr virus-induced human lymphoblastoid cell lines (LCL). The ADCC activity with PLB sensitized by HLA-A or HLA-B antibodies was relatively low, but higher than that of control. DR antibodies completely failed to sensitize PLB for ADCC. LCL sensitized by corresponding DR antibodies gave strong ADCC reactions, independent from their D phenotypes. The ADCC reactions against LCL were abolished by absorption of the DR antibodies from the alloantisera. Thus the ADCC with LCL would provide a procedure to study the role of DR antibodies in chronic rejection of long-term renal allografts.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: International archives of allergy and applied immunology
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
