Abstract
This study proposes a microfluidic device used for X-/Y-sperm separation based on monoclonal antibody-conjugated magnetic beads, which become positively charged in the flow system. Y-sperms were selectively captured via a monoclonal antibody and transferred onto the microfluidic device and were discarded, so that X-sperms can be isolated and commercially exploited for fertilization demands of female cattle in dairy industry. Therefore, the research team used monoclonal antibody-conjugated magnetic beads to increase the force that causes the Y-sperm to be pulled out of the system, leaving only the X-sperm for further use. The experimental design was divided into the following: Model 1, the microfluid system for sorting positive magnetic beads, which yielded 100% separation; Model 2, the sorting of monoclonal antibody-conjugated magnetic beads in the fluid system, yielding 98.84% microcirculation; Model 3, the sorting of monoclonal antibody-conjugated magnetic beads with sperm in the microfluid system, yielding 80.12% microcirculation. Moreover, the fabrication microfluidic system had thin film electrodes created via UV lithography and MWCNTs electrode structure capable of erecting an electrode wall 1500 µm above the floor with a flow channel width of only 100 µm. The system was tested using a constant flow rate of 2 µL/min and X-/Y-sperm were separated using carbon nanotube electrodes at 2.5 V. The structure created with the use of vertical electrodes and monoclonal antibody-conjugated magnetic beads technique produced a higher effective rejection effect and was able to remove a large number of unwanted sperm from the system with 80.12% efficiency.
Highlights
Industrial Engineering Department, Faculty of Engineering, Chiang Mai University, Animal and Aquatic Science Department, Faculty of Agriculture, Chiang Mai University, Abstract: This study proposes a microfluidic device used for X-/Y-sperm separation based on monoclonal antibody-conjugated magnetic beads, which become positively charged in the flow system
To increase the performance of sperm sorting based on electrophoresis technique, we present lab on a chip (LOC) integrated with multiwall carbon nanotube (MWCNT) electrodes, which provides the possibility for increasing the performance both effectiveness and productivity
The micro-fluid system and micro-electrodes were created for separating magnetic particles beads, monoclonal antibodies conjugated with magnetic particle beads and sperm and sperm with monoclonal antibodies conjugated magnetic particle beads with the device
Summary
Industrial Engineering Department, Faculty of Engineering, Chiang Mai University, Animal and Aquatic Science Department, Faculty of Agriculture, Chiang Mai University, Abstract: This study proposes a microfluidic device used for X-/Y-sperm separation based on monoclonal antibody-conjugated magnetic beads, which become positively charged in the flow system. The research team used monoclonal antibody-conjugated magnetic beads to increase the force that causes the Y-sperm to be pulled out of the system, leaving only the X-sperm for further use. The structure created with the use of vertical electrodes and monoclonal antibody-conjugated magnetic beads technique produced a higher effective rejection effect and was able to remove a large number of unwanted sperm from the system with 80.12% efficiency. It was shown that the motility of X and Y chromosome-containing sperm cells differs under a magnetic field, where the female sperm cell is faster than the male. The conventional centrifugation based on swim-up (CSW) and direct swim-up (DSW) play roles in non-complicated method but comprise various centrifugation steps and oxidative stress, resulting in sperm
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